2015 Fiscal Year Final Research Report
Development of bioluminescent probe for small compound based on BRET
| Project/Area Number |
25350983
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Chemical biology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
WU CHUN 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (90415646)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 発光プローブ / ペプチド / ファージ / 生物発光共エネルギー移動 / ルシフェラーゼ / 蛍光タンパク質 |
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| Outline of Final Research Achievements |
In this study, we tried to develop an optical probe for imaging small molecule by using bioluminescence resonance energy transfer (BRET) method and phage display technique. First, two peptides which have affinity for S-adenosyl-homocysteine were selected from phage library. Then the linker peptide between the selected peptides were optimized.The DNA coding the selected peptide was inserted to the DNA construct containing gaussia luciferase and green fluorescent protein genes. Finally the detection of SAH was examined by monitoring of the BRET signal of the fusion protein.
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| Free Research Field |
ケミカルバイオロジー
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