2015 Fiscal Year Final Research Report
Excitatory synaptic transmission is regulated by the number of regional astrocytes in single cultured hippocampal neuron
| Project/Area Number |
25350988
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Basic / Social brain science
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA YASUO 東京工科大学, 工学部, 教授 (40312673)
MA TENG 東北大学, 電気通信研究所, 助教 (10734543)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | アストロサイト / シナプス伝達 / グリア |
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| Outline of Final Research Achievements |
Neural circuit is composed of mainly neuron and glial cell. Astrocyte is a major glial cell which plays key roles in brain function including formation and maturation of synapses. In this study, we utilized primary co-culture system of autaptic single hippocampal neuron with dot-cultured cortical astrocytes, where experimentally modified the density of astrocytes. Excitatory synaptic transmission was decreased by lower density of astrocytes (the number of astrocytes = 1-5), which was accompanied by a decrease of readily releasable pool of synaptic vesicles but not of the vesicular release probability. Distribution analysis of synaptic puncta stained with FM styryl dye and VGLUT-1 antibody revealed that a proportion of presynaptically silent synapses on dendrites was increased by a small density of astrocytes. These findings indicate that synaptic transmission and a position of presynaptically active synapse are varied by the number of astrocytes surrounding neuron.
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| Free Research Field |
神経生理学
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