2015 Fiscal Year Final Research Report
Controlling the higher-order structure of genomic DNA in relation to its genetic activity
Project/Area Number |
25390038
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Nanobioscience
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Research Institution | Ritsumeikan University |
Principal Investigator |
YOSHIKAWA Yuko 立命館大学, 総合科学技術研究機構, 教授 (80291871)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 長鎖DNA / 単一分子観察 / 高次構造 / 二重鎖切断 |
Outline of Final Research Achievements |
Through the use of single-molecule observation with fluorescence microscopy, we studied the higher-order structural change of a giant DNA molecule. Results obtained are as follows. (1) The effects of linear, branched and cyclic polyamines on the higher-order structure of giant DNA were examined. These polyamines showed distinct activities, thus highlighting the importance of polyamine backbone structure. (2) The protective effect of ascorbic acid (AsA) against double-strand breaks in DNA was evaluated. Samples were exposed to three different irradiations: visible light, gamma-ray and ultrasound. It was found that AsA had almost no protective effect against the damage caused by ultrasound.
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Free Research Field |
生物物理化学
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