Modelling hydration structure of nucleic acid using corresponding gas-phase hydrated clusters: Toward elucidating nucleotide structure

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410023
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Yokohama City University
• Principal Investigator: Saigusa Hiroyuki 横浜市立大学, 生命ナノシステム科学研究科, 教授 (90162180)
• Co-Investigator(Kenkyū-buntansha): TSUKAMOTO Masaki 名古屋大学, 情報科学研究科, 助教 (10362295)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 核酸塩基 / 水和クラスター / 中赤外レーザー / 尿酸 / ローカルモード結合

Outline of Final Research Achievements

In order to model the hydration structure of nucleic acid systems in relation to their biological functions, we have performed structural characterization of corresponding hydrated clusters isolated in the gas-phase. We have been able to establish an effective strategic method for elucidating hydration structure of nucleotides, in which a sugar-phosphate group is bonded to the base.
With this financial support, we have also developed a novel Mid-IR spectroscopic system and measured CO stretching vibrational spectra of uric acid and its monohydrates.It was found that the two structural isomers of the monohydrated cluster show very different local mode-coupling behavior, depending strongly upon the hydrogen-bonding site. By using this mode coupling scheme, we have been able to identify unambiguously the hydrogen-bonded structure of uric acid-melamine complex.

Free Research Field

レーザー分光学および光化学