2015 Fiscal Year Final Research Report
Modelling hydration structure of nucleic acid using corresponding gas-phase hydrated clusters: Toward elucidating nucleotide structure
Project/Area Number |
25410023
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Physical chemistry
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Research Institution | Yokohama City University |
Principal Investigator |
Saigusa Hiroyuki 横浜市立大学, 生命ナノシステム科学研究科, 教授 (90162180)
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Co-Investigator(Kenkyū-buntansha) |
TSUKAMOTO Masaki 名古屋大学, 情報科学研究科, 助教 (10362295)
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Project Period (FY) |
2013-04-01 – 2016-03-31
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Keywords | 核酸塩基 / 水和クラスター / 中赤外レーザー / 尿酸 / ローカルモード結合 |
Outline of Final Research Achievements |
In order to model the hydration structure of nucleic acid systems in relation to their biological functions, we have performed structural characterization of corresponding hydrated clusters isolated in the gas-phase. We have been able to establish an effective strategic method for elucidating hydration structure of nucleotides, in which a sugar-phosphate group is bonded to the base. With this financial support, we have also developed a novel Mid-IR spectroscopic system and measured CO stretching vibrational spectra of uric acid and its monohydrates.It was found that the two structural isomers of the monohydrated cluster show very different local mode-coupling behavior, depending strongly upon the hydrogen-bonding site. By using this mode coupling scheme, we have been able to identify unambiguously the hydrogen-bonded structure of uric acid-melamine complex.
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Free Research Field |
レーザー分光学および光化学
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