Study toward a practical method for protein structure analysis using an artifact-free fluorescence-detected circular dichroism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410044
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Organic chemistry
• Research Institution: Hiroshima University
• Principal Investigator: Nehira Tatsuo 広島大学, 総合科学研究科, 准教授 (60321692)
• Co-Investigator(Renkei-kenkyūsha): ISHIDA ATSUHIKO 広島大学, 大学院総合科学研究科, 教授 (90212886) ; YAMAZAKI TAKESHI 広島大学, 大学院総合科学研究科, 教授 (30192397)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: タンパク質 / 円二色性 / 蛍光

Outline of Final Research Achievements

Aiming at a practical protein structure analysis with fluorescence-detected CD, structure-spectrum relationship was investigated by using a synthetic library of oligopeptides that contain Trp as a fluorescent amino acid. Trp was derivatized systematically with a variety of oligoprolines as rigid spacers. As a result of careful comparison among those FDCD spectra, it was inferred from the expansion at Trp C-terminal that observable are two interactions, the coupling of Trp with neighboring peptides and the energy transfer from Phe to Trp over the spacer. On the other hand, the expansion at Trp N-terminal implied that the coupling with neighboring peptides would be suppressed. A micro-scale cell that requires only a tenth of volumes was also developed.

Free Research Field

構造有機化学