Carbon Fixation Reactions Modeling Iron/Nickel Enzymes Included in Acetyl CoA Pathway

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Inorganic chemistry
• Research Institution: Nagoya University
• Principal Investigator: Matsumoto Tsuyoshi 名古屋大学, トランスフォーマティブ生命分子研究所, 准教授 (50311717)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ニッケル酵素 / アセチルCoA合成酵素 / COデヒドロゲナーゼ / 炭素固定 / モデル研究

Outline of Final Research Achievements

Model studies of CO dehydrogenase (CODH) and acetyl CoA synthase (ACS) were conducted to elucidate the unrevealed mechanism of the carbon fixation process of acetyl CoA pathway in nature. As a CODH active site model, a novel [4Fe-5S-Ni] cluster was synthesized by utilizing our unique tridentate thiolate ligands. For our model studies of ACS, a series of dinuclear nickel complexes having tetra-anionic diamido-dithiolate ligand “mbea” modeling the plausible intermediates included in the ACS catalytic pathway was synthesized, and the thioester formation reaction modeling the ACS was accomplished. The results considerably contribute to elucidate the ACS reaction mechanism, and provide clues to the development of new carbon fixation reactions.

Free Research Field

生物無機化学