Development of Sugar Sensing Nanoparticles with Signal Amplification Based on Swelling of hydrogel

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410141
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: University of Toyama
• Principal Investigator: TOHDA Koji 富山大学, 大学院理工学研究部(工学), 教授 (60212065)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: オプティカルセンサー / 血糖値 / グルコースレセプター / ビスベンゾボロキソール / 高分子架橋点 / ポリマー膨潤

Outline of Final Research Achievements

In order to develop subdermally implantable devices for continuous blood glucose monitoring, we fabricated optical glucose sensing films with the semi-interpenetrating polymer network (semi-IPN) structure composed of glucose receptor-attached polymers and receptor specific dye-attached polymers, in which the receptor/dye complexes act as crosslinking points. The glucose sensing principle is based on the competitive binding of the receptor between the dye and glucose, providing a swelling of the sensing film due to the decrease in the number of crosslinking points in the polymer network: the swelling facilitates further dissociation of the receptor/dye complexes, leading to amplification of sensor color responses. The thus prepared sensing films showed remarkable absorbance responses for glucose (around 20% absorbance change in physiological glucose level) with reversible polymer swelling and shrinking.

Free Research Field

分析化学