Construction of analytical systems in fibrillation of target protein

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Ehime University
• Principal Investigator: Shimazaki Yoji 愛媛大学, 理工学研究科, 准教授 (80284389)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 分離分析 / 抗体固定化膜 / アミロイドβ / 微量分析 / 生体試料分析

Outline of Final Research Achievements

The fibrillation of an amyloid β (Aβ) monomer is related to the progression of Alzheimer’s disease. For the examination of Aβand its fibrous form, a method of isolation and analysis in Aβand its fibrous form is important. In the present study, a method of Aβmonomer isolation from human plasma with the Aβ was established using membrane-immobilized anti-Aβantibody (immuneaffinity membrane). We indicated that this method could be used to capture and analyze Aβwithin the micro-space such as nasal cavity, and the fibrillation of Aβ could be suppressed via protease after Aβ trapping. In addition, fibrous form of Aβ could be isolated from the spiked human plasma by a combinational method of immuneaffinity membrane and electrophoresis.

Free Research Field

分析化学