2016 Fiscal Year Final Research Report
Advanced design and application of an electrochemical sensor for monitoring of glutamate in brain slices
| Project/Area Number |
25410149
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | L-グルタミン酸 / 電気化学センサー / ｆEPSP / 急性脳スライス / 神経伝達物質 / 可視イメージング / バイオセンシング |
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| Outline of Final Research Achievements |
An obstacle for the simultaneous monitoring of field excitatory postsynaptic potentials (fEPSPs) and glutamate currents at a glass capillary-based L-glutamate sensor was investigated. The proposed protocols were tested to acquire Faradaic currents at the region CA1 of mouse hippocampal slices and showed that the present approach is a useful technique knowing L-glutamate level at CA1 region in brain slices. We showed an evidence that the release of glutamate is enhanced after the induction of long-term potentiation in the presence and absence of an AMPA receptor CNQX. Also, we proposed a new method for chemical imaging of extracellular L-glutamate in a flow system.
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| Free Research Field |
分析化学
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