2015 Fiscal Year Final Research Report
Development of functional biochips with nano-scale layered structure for sensitive proteomics
| Project/Area Number |
25410151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Technology |
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Principal Investigator |
YANO Kazuyoshi 東京工科大学, 応用生物学部, 教授 (40262109)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | プロテオミクス / プラズマ重合 / 分析化学 / イムノアッセイ / 蛍光 / 薄膜 |
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| Outline of Final Research Achievements |
Nano-scale layered structure was fabricated on a 96-well microplate modified with silver layer as a metal mirror and with plasma-polymerized film as an optical interference layer. Fluorescence-based sandwich immunoassay targeting C-reactive protein and interleukin-2 was performed using the microplate. It was shown that enhanced fluorescence signal was obtained with the modified microplate compared with the unmodified one, resulting in highly sensitive detection.
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| Free Research Field |
分析化学、分子生物学
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