Development of fluorescent dyes in the near-infrared region for bio-analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410152
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Analytical chemistry
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: SASAKI Shin-ichi 長浜バイオ大学, バイオサイエンス学部, 准教授 (50317294)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Koji 慶應義塾大学, 理工学部, 教授 (80154540) ; TAMIAKI Hitoshi 立命館大学, 薬学部, 教授 (00192641)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 蛍光 / 分子認識 / 超分子 / 光合成

Outline of Final Research Achievements

Aiming at the analytical applications in the field of biochemistry, functional fluorescent dyes were synthesized based on naturally occurring (bacterio)chlorophylls. 1) The synthetic C3-ureidochlorin showed fluorescence emission spectral responses with the addition of anions in dichloromethane, 2) a series of carboxylated chlorins were synthesized and the detection of additional amine in THF was demonstrated by both visible absorption and fluorescence emission spectroscopies, 3) the selective detection of histidine was demonstrated using monomeric/oligomeric zinc chlorins in an aqueous buffer solution, and 4) bacteriochlorophyll-a derivatives possessing different substituents at the C13-position were synthesized, which showed fluorescence emission maxima in the longer wavelength region (>800nm).

Free Research Field

化学