2016 Fiscal Year Final Research Report
Analysis of beat fluctuations in cardiomyocytes by scanning electrochemical microscopy
| Project/Area Number |
25410162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
Hirano Yu 国立研究開発法人産業技術総合研究所, 生物プロセス研究部門, 主任研究員 (70415735)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 電気化学 / 走査型電気化学顕微鏡 / 心筋細胞 / 一細胞分析 / マイクロ電極 |
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| Outline of Final Research Achievements |
Analysis of cardiac action potential is currently used to evaluate the pharmacological and toxicological properties of drugs. In this study, we developed a SECM system for analyzing contraction kinetics and energy metabolisms in cardiomyocytes. To analyze a glucose consumption in living cells, multiple enzymes were immobilized on a microelectrode surface by attachment at the termini of various double strands DNA. The enzyme immobilized microelectrode could be used for imaging glucose distributed in a local area.
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| Free Research Field |
生物電気化学
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