Novel Polymer Nanospheres for Serological Diagnosis of Malaria

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Gunma University
• Principal Investigator: Oku Hiroyuki 群馬大学, 大学院理工学府, 准教授 (20301749)
• Co-Investigator(Renkei-kenkyūsha): YANO Kazuhiko 国立研究開発法人国立国際医療研究センター, 研究所, 上級研究員 (30392393) ; KANO Shigeyuki 国立研究開発法人国立国際医療研究センター, 研究所, 部長 (60233912)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 微粒子 / 高分子 / ペプチド / 抗原 / マラリア / 血清 / 抗体価 / 生体材料

Outline of Final Research Achievements

Malaria is a major public health problem especially in tropical and sub-tropical regions of the world. The methods most commonly used to diagnose malaria patients sera, such as IFAT and ELISA, are not easy methods to examine in the bedside and the clinical laboratory. Here, we wish to report novel diagnostic nanospheres for the detection of specific antibodies in malaria patients’ sera.
The peptide antigen was designed from a Plasmodium specific sequence in lactate dehydrogenase. The test-material is prepared by chemical coupling of the peptides to the surface of the nanosphere.
We have compared the reactivity against malaria patients’ sera collected in endemic regions of the Philippines: (a) Mixed infected-, (b) falciparum-, (c) vivax-malaria patients, and (d) feverish patients (malaria-negative). Successful results were observed for the nanosphere material, which showed good specificity for three kinds of malaria patients compared with feverish patients.

Free Research Field

生体関連科学