2015 Fiscal Year Final Research Report
Creation of novel poly-extremozymes by modifying protein surfaces
| Project/Area Number |
25410172
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
|
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 極限環境微生物 / 極限酵素 / 分子表面電荷 / タンパク質工学 / 進化分子工学 / 極限環境耐性 / 比活性 |
|---|
| Outline of Final Research Achievements |
Alkalitolerancy, thermotolerancy and halotolerancy of a GH family 11 alkaline xylanase, XynJ, were improved by introducing basic amino acids on its protein surface. Halotolerancy and organic solvent-tolerancy of a GH family 18 halotolerant chitinase, ChiN1, were improved by introducing acidic amino acids on its protein surface. Alkalitolerancy and specific activity of a GH family 10 hyper-thermotolerant xylanase, XynTB, were improved by directed evolution.
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| Free Research Field |
生体関連化学
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