Total chemical synthesis of ubiquitinated glycoportein for the study of glycoprotein degradation process

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410175
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Bio-related chemistry
• Research Institution: Osaka University
• Principal Investigator: Izumi Masayuki 大阪大学, 理学(系)研究科(研究院), 准教授 (80332641)
• Co-Investigator(Kenkyū-buntansha): KAJIHARA Yasuhiro 大阪大学, 大学院理学研究科, 教授 (50275020)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 糖タンパク質 / 化学合成 / ユビキチン / 分解経路

Outline of Final Research Achievements

Misfolded glycoproteins are toxic to the cell and are thought to be degraded by ubiquitin-proteasome system (UPS), however, the detail of the degradation pathway is not clearly known. In this study, I accomplished the total synthesis of ubiquitinated glycoprotein, and used it as a probe for the study of the degradation of glycoprotein by UPS. This is one of the very few example of the total synthesis of posttranslationally modified complex protein having oligosaccharide and ubiquitin. The degradation assay suggested that the newly developed synthetic ubiquitinated glycoprotein probe can be a valuable tool for the analysis of the degradation of glycoproteins by UPS.

Free Research Field

生体分子化学