2015 Fiscal Year Final Research Report
Development of pH-responsive fluorescent nucleosides for SNPs detection
| Project/Area Number |
25410180
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Bio-related chemistry
|
|---|
| Research Institution | Nihon University |
|---|
Principal Investigator |
SAITO Yoshio 日本大学, 工学部, 准教授 (40385985)
|
|---|
| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 蛍光プローブ |
|---|
| Outline of Final Research Achievements |
We have synthesized various pH-responsive fluorescent deoxyuridine derivatives containing pyridine skeleton. These fluorescent nucleosides exhibited distinctive fluorescence at 470-550 nm in aqueous solvents at basic to neutral pH values. Interestingly, 2-aminopyridine derivative exhibited strong fluorescence emission at 470 nm at pH range of 1.6-3.0 with a pKa value of 3.4 whereas exhibited emission at 550 nm at pH > 4.0. Such pH-sensitive fluorescent nucleosides can be used as fluorescence switch for monitoring pH change in biological systems.
|
| Free Research Field |
生物有機化学
|
