2015 Fiscal Year Final Research Report
Exploration of optimized chemical structure of siRNA based on the functional structure of human argonaute 2 protein
| Project/Area Number |
25410182
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Bio-related chemistry
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| Research Institution | Kinki University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 核酸化学 / 遺伝子サイレンシング / 化学修飾siRNA / アルゴノート2 |
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| Outline of Final Research Achievements |
In the present study, we could demonstrate that chemical modification of siRNA increased the probability of the selection of antisense strand by Ago2, that conjugation of siRNA with signal peptides made it possible to control the intracellular localization of siRNA, and that non-covalent complexation of siRNA with the designed amphiphilic peptide improved cellular uptake and biological stability of siRNA. By a combination of further chemical modification, conjugation and complexation with a variety of functional molecules, we believe we can design an optimized structure of siRNA therapeutics which can effectively penetrate into cells and bind to the target molecule with a good affinity and specificity, without any side effects.
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| Free Research Field |
化学
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