Enzymatic oxidation and reduction using risk-free and naturally existing oxidizing or reducing agents and carbon dioxide

2015 Fiscal Year Final Research Report

• Project/Area Number: 25410189
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Green/Environmental chemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: Matsuda Tomoko 東京工業大学, 生命理工学研究科, 准教授 (10319494)
• Co-Investigator(Kenkyū-buntansha): YAMANAKA Rio 姫路獨協大学, 薬学部, 講師 (40454764)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: グリーンケミストリー / 酵素

Outline of Final Research Achievements

The oxidation and reduction reactions with risk-free reaction conditions were developed. The overexpressed enzymes (oxidase from Fusarium and reductase from Geotrichum candidum) using E-coli were used for the reactions, resulting in the very high efficiencies. Both enzymes have very wide substrate specificities, and excellent enantioselectivities. Especially, biotransformations of the challenging substrates for asymmetric synthesis were found to be also successful. Moreover, the structure of the G. candidum reductase was investigated by modeling and X-ray crystal structure determination to examine the mechanism to succeed in the excellent enantioselectivity.

Free Research Field

グリーン・環境化学