Mechanical Evaluation for Articular Cartilage Based on Molecular Structure Analysis Using Raman Spectroscopy

2015 Fiscal Year Final Research Report

• Project/Area Number: 25420001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Materials/Mechanics of materials
• Research Institution: Hokkaido University
• Principal Investigator: TODOH MASAHIRO 北海道大学, 工学(系)研究科(研究院), 准教授 (10314402)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: バイオメカニクス / ラマン分光 / 関節軟骨 / コラーゲン / プロテオグリカン

Outline of Final Research Achievements

In this study, component analysis as mechanical evaluation was performed by Raman spectroscopy for cartilage consisted by macromolecule (such as collagen fiber and proteoglycan). Raman spectroscopy is a vibrational spectroscopy by Raman scattering, and allows simultaneous measurement of organic and inorganic components. The spectroscopy is suitable for measurement of biological samples because of little effect on water. The cartilage specimens were cut out from bovine knee joint and their Raman spectrum were analyzed under compressive loading. In addition, load response to cartilage by the spectroscopy. The results of Raman shift for hydroxyproline, proline, phenylalanine and Amide I show the mechanical response. Therefore, this study indicated the possibility of stress analysis for cartilage with Raman spectroscopy

Free Research Field

バイオメカニクス