Quantitative evaluation of cell dynamics by pulsed electric field treatment and establishment of cell diagnostic system by dielectrophoresis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25420412
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: Tokyo Metropolitan University
• Principal Investigator: Uchida Satoshi 首都大学東京, 理工学研究科, 准教授 (90305417)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 計測システム / 誘電泳動 / パルス電界 / 細胞動態 / 診断

Outline of Final Research Achievements

In the present work, we investigated the changes in permittivity and conductivity of the yeasts imposed by pulsed electric field with dielectrophoretic velocimetry. The relationship between interelectrode impedance due to integrated cells and the activities of membrane and growth was verified quantitatively. We also tried to establish a rapid and simple diagnostic system of cell dynamics.
As a major achievement, we quantified the damage degree of membrane using nucleic acid staining, and identified the difference of threshold against heating and applying of pulsed electric field. The electrical constants were deduced from the equivalent electrical circuit model in the comparison with dielectrophoretic velocity of yeast cells and interelectrode impedance. Further, the gene expression of damaged yeasts was detected and the judgment indicators of biological mutation were decided. In addition, the detection responses were reviewed under various pulse conditions.

Free Research Field

静電気学、計測・センサ工学、放電物理