Egg-based oral immunotherapy using genetically manipulated chickens

2015 Fiscal Year Final Research Report

• Project/Area Number: 25420834
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyushu University
• Principal Investigator: Kawabe Yoshinori 九州大学, 工学(系)研究科(研究院), 助教 (30448401)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 遺伝子導入ニワトリ / アレルゲンエピトープ / エピトープペプチド / スギ花粉症 / ワクチン卵 / M細胞 / 経口免疫ワクチン / MHC

Outline of Final Research Achievements

Here, we attempted to develop egg-based oral immunotherapy using genetically manipulated (GM) chickens. By using egg white derived from GM hens producing human T-cell epitopes fused with MHC molecules (MHC-Crp-Fc), the number of sneezing and IgE level were significantly decreased in allergic mice fed with MHC-Crp-Fc-containing egg white. TGF-beta1 could be produced in an oviduct-specific manner. Recombinant RANKL that can induce M cells was produced. Eggs derived from GM chickens may be served as edible pharmaceuticals for allergy immunotherapy.

Free Research Field

遺伝子工学