2015 Fiscal Year Final Research Report
Development of a molecular connection method for generating highly functional antibodies
| Project/Area Number |
25420836
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Osaka City University |
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Principal Investigator |
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| Project Period (FY) |
2013-04-01 – 2016-03-31
|
| Keywords | 抗体 / 酵素 / 受容体 / タンパク質工学 / 分子認識 |
|---|
| Outline of Final Research Achievements |
In this study, we used a bacterial expression system to produce a bispecific antibody that was heterotetramerized through hetero-associating peptides and that targeted cancer cells. As the results, we succeeded in preparing the recombinant antibody which bound to two target molecules simultaneously. Furthermore, the bispecific antibody showed cytotoxic activity against target tumor cells, and its cytotoxicity was comparable to that of a commercial therapeutic antibody. These results show that our method has high potential for the cost-efficient production of highly functional antibodies.
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| Free Research Field |
タンパク質工学
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