Development of biosensor for evaluation of irreversible enzyme inhibitor and application of the sensor for screening of the reagents

2015 Fiscal Year Final Research Report

• Project/Area Number: 25420841
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biofunction/Bioprocess
• Research Institution: Kanagawa Institute of Technology
• Principal Investigator: IIDA YASUHIRO 神奈川工科大学, 応用バイオ科学部, 教授 (40329305)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: セクレターゼ / 固定化酵素 / 阻害剤

Outline of Final Research Achievements

We developed evaluation system for activity of beta-secretase (BACE1) by using orientated immobilized recombinant BACE1 in combination with a FIA (Flow Injection Analysis) system. We tried to miniaturize to the system for minimize of material and reduce assay time by micro flow channel. And, we developed fusion protein which is made from ECFP, EYFP, and those linker including BACE1 recognition sequence as a novel FRET (Fluorescence Resonance Energy Transfer) substrate and evaluate the properties. This protein has advantage not only in cost effective screening for BACE1 inhibitor but in in vivo assay for BACE1 activity. Moreover, we applied the developing system to evaluate BACE1 inhibitor. After that, the system was used as a screening method for BACE1 inhibitor from herbal medicine and novel synthesized chemicals. Read compound of BACE1 inhibitor was obtained by the screening.

Free Research Field

バイオセンサ