2015 Fiscal Year Final Research Report
Development of novel glycan probe for monitoring molecular chaperone activity.
Project/Area Number |
25420844
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biofunction/Bioprocess
|
Research Institution | University of Toyama (2014-2015) Institute of Physical and Chemical Research (2013) |
Principal Investigator |
SAKONO MASAFUMI 富山大学, 大学院理工学研究部(工学), 准教授 (50391959)
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | 分子シャペロン / レクチン / 糖鎖 |
Outline of Final Research Achievements |
NileRed derivatives combined with spacer were synthesized. These derivatives were connected to amidated glycan, resulting that glycan which has environmental responsible ability. Recombinant human CRT was produced by E. coli. A cDNA encoding human CRT was cloned into pCold I expression plasmid , which was designed to produce N-terminally (His)6-tagged proteins.
|
Free Research Field |
タンパク質工学
|