Analysis of axon collaterals of cortical projection neurons by Tet double infection (TEDI) method

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430018
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurophysiology / General neuroscience
• Research Institution: Institute of Physical and Chemical Research (2015); National Institute for Basic Biology (2013-2014)
• Principal Investigator: Akiya Watakabe 国立研究開発法人理化学研究所, 脳科学総合研究センター, 研究員 (40290910)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 大脳皮質 / 逆行性 / 神経解剖学 / マーモセット / ウイルスベクター / 投射ニューロン / 視床 / 層構造

Outline of Final Research Achievements

In this project, I endeavored to establish and further polish up my new viral technology termed TEDI (TET double Infection method), in which retrograde and anterograde viral vectors were combined to selectively transduce a particular type of projection neurons. Using this technique, I was able to selectively visualize and analyze the morphologies of the axon collaterals of the corticothalamic and corticocortical neurons in mice. I also examined the infection conditions of various serotypes of AAV in marmosets, which result is useful in planning an efficient TEDI in non-human primates.

Free Research Field

神経生物学