Investigation of mechanisms of dendritic differentiation of cerebellar Purkinje cells mediated by ryanodine receptors

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430040
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Nerve anatomy/Neuropathology
• Research Institution: Nagoya City University
• Principal Investigator: Tanaka Masahiko 名古屋市立大学, 薬学研究科(研究院), 准教授 (60267953)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: リアノジン受容体 / カルシウム / プルキンエ細胞 / 樹状突起 / 小脳 / siRNA / 単一細胞エレクトロポレーション / CaM kinase

Outline of Final Research Achievements

We investigated mechanisms of dendritic differentiation of cerebellar Purkinje cells mediated by ryanodine receptors (RyRs). Using single-cell electroporation of siRNA and calcium imaging, we revealed that RyR type 1 expressed by Purkinje cells promotes dendritic differentiation of Purkinje cells through phosphorylation of the common substrates of calcium/calmodulin-dependent protein kinase (CaMK) IIa, IIb and IV. On the other hand, RyR type 2 expressed by granule cells was also found to promote dendritic differentiation of Purkinje cells through secretion of brain-derived neurotrophic factor (BDNF) from granule cells.

Free Research Field

分子神経生物学