2015 Fiscal Year Final Research Report
Evaluation of prion protein oligomerization and abnormality on human prion diseases
| Project/Area Number |
25430050
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | Kyushu University |
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Principal Investigator |
HONDA HIROYUKI 九州大学, 医学(系)研究科(研究院), 助教 (90624057)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | プリオン病 / プリオン蛋白 / オリゴマー / ゲル濾過 / 遠心カラム |
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| Outline of Final Research Achievements |
We used size exclusion gel chromatography to evaluate oligomeric prion protein on some human prion diseases. In all examined samples (sporadic Creutzfeldt-Jakob disease (CJD), CJD with E200K mutation, CJD with V180I mutation, Gerstmann-Straussler-Scheinker disease (P102L), D178fs25 Prion disease), oligomeric fraction contained a lot of proteinase K resistant prion protein (PrPres), respectively. Low molecular PrPres detected in some kinds of prion diseases were also contained in the oligomeric fraction. Our results suggested that PrPres tends to form oligomeric state.
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| Free Research Field |
総合領域
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