The formation of GABAergic network and rhyhm of repiratory in the embryonic development

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430066
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: University of the Ryukyus
• Principal Investigator: SHIMIZU Chigusa 琉球大学, 医学(系)研究科(研究院), 特命助教 (70435072)
• Co-Investigator(Kenkyū-buntansha): OKABE Akihito 琉球大学, 大学院医学研究科, 准教授 (10313941)
• Co-Investigator(Renkei-kenkyūsha): TAKAYAMA Chitoshi 琉球大学, 大学院医学研究科, 教授 (60197217) ; YANAGAWA Yuchio 群馬大学, 大学院医学研究科, 教授 (90202366)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: GABA / KCC2 / hypogrossal

Outline of Final Research Achievements

GABA plays important roles in the respiratory rhythm. Role of GABAergic transmission in regulation of medullary respiration-related rhythmic activity (RRA) perinatally, however is yet to be determined. In this study the numbers of RRA were significantly increased in embryonic development but there were no significant changes after birth. VGAT transports GABA to synaptic vesicles. GABAergic action is changed from excitatory to inhibitory by expression of KCC2. Both VGAT and KCC2 deficient mice die in their birthday by respiratory failure. By compared these mice, it found that exocytosis of GABA is necessary to generate RRA, however KCC2 needs for making of rhythm of respiratory. .

Free Research Field

神経生化学・神経解剖学