Establishment of freezing method of rabbit embryos for cryopreservation of laboratory rabbits as human disease models

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430091
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Saga University
• Principal Investigator: KITAJIMA Shuji 佐賀大学, 総合分析実験センター, 准教授 (70284643)
• Co-Investigator(Kenkyū-buntansha): NISHIJIMA Kazutoshi 秋田大学, バイオサイエンス教育研究センター, 准教授 (70435874) ; MATSUHISA Fumikazu 佐賀大学, 総合分析実験センター, 助教 (20754253)
• Co-Investigator(Renkei-kenkyūsha): FAN Jianglin 山梨大学, 医学工学総合研究部, 教授 (60272192) ; MATSUDA Yukihisa 秋田大学, バイオサイエンス教育研究センター, 准教授 (50157327)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 凍結保存 / バイオリソース / ウサギ / 疾患モデル

Outline of Final Research Achievements

In recent years, cryopreservation by spermatozoa and embryos become an important technique to preserve strains of the laboratory animals as human disease models. In this study, we found that rabbit morula stage embryos vitrified by combination of FES (Ficoll and ethylene glycol in sucrose buffer) and special device (Cryotop) shown good in vitro development rate equally to that of non-freezing embryos. In addition to establishment of cryopreservation method in rabbit embryos, we could do endoscopic embryo transfer technique which is also necessary to preserve useful laboratory rabbit strains as human disease models.

Free Research Field

実験動物学