2016 Fiscal Year Final Research Report
Sexually dimorphic gene expression in exchangeable gene trap mouse lines
| Project/Area Number |
25430092
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Kumamoto University |
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Principal Investigator |
Yoshinobu Kumiko 熊本大学, 生命資源研究・支援センター, 助教 (20274730)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 性差発現 / 可変型遺伝子トラップ法 / X-gal染色 / 表現型解析 / Elovl6遺伝子 / EGTCデータベース |
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| Outline of Final Research Achievements |
We performed gene expression profiling in gene trap mouse lines and found sexual dimorphic genes. RT-PCR using cDNA of C57BL/6(B6) strain showed a slight difference between male and female in the expression of Smyd2, Tcf12 and Elovl6.
In phenotype analysis of the Elovl6 gene trap mouse, homozygous mice were born at the expected Mendelian ratio. However, body weight in homozygous mice was lower than wild-type mice and heterozygous mice, and the difference was remarkable in male. qRT-PCR using cDNA of B6 strain showed no difference between male and female in Elovl6 expression. It was suggested that there was the sexual dimorphism of Elovl6 expression in protein level.
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| Free Research Field |
発生生物学、分子生物学
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