Development of mouse models for heteroplasmic mitochondrial DNA transmission

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: SHITARA Hiroshi 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 主任基盤技術研究職員 (90321885)
• Co-Investigator(Kenkyū-buntansha): YONEKAWA Hiromichi 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 特任研究員 (30142110) ; YAMAGUCHI Midori 公益財団法人東京都医学総合研究所, 基盤技術研究センター, 基盤技術研究職員 (20593643)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: ミトコンドリア / ミトコンドリアDNA / ヘテロプラズミー

Outline of Final Research Achievements

Mitochondrial DNA (mtDNA) heteroplasmy is the presence of two or more types of mtDNA molecules within single cells and individuals. To investigate the mode of heteroplasmic mtDNA transmission in mammals, heteroplasmic mtDNA mouse strains were established using cytoplasmic transfer methods by microinjection. The proportion of heteroplasmic mtDNA was measured by PCR-RFLP methods, which were improved to eliminate the influence of artifacts. Moreover, a knockout mouse strain was established using CRISPR/Cas9 system to investigate the target gene function associated with the inheritance of heteroplasmic mtDNA.

Free Research Field

実験動物学