Development of new therapeutic strategy targeting epithelial-mesenchymal transition and sialyl lewis glycans

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430129
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor biology
• Research Institution: Aichi Cancer Center Research Institute
• Principal Investigator: Sakuma Keiichiro 愛知県がんセンター(研究所), 分子病態学部, 主任研究員 (90402891)
• Co-Investigator(Renkei-kenkyūsha): AOKI Masahiro 愛知県がんセンター(研究所), 分子病態学部, 部長 (60362464) ; KANNAGI Reiji 愛知医科大学, 先端医学研究センター, 客員教授 (80161389)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 上皮間葉転換

Outline of Final Research Achievements

The object of this study is to establish a new therapeutic strategy for colon cancer through elucidating the molecular mechanism underlying c-Myc-induced sialyl lewis glycan expression in colon cancer cells undergoing epithelial-mesenchymal transition (EMT). Identification of the EMT-specific modification of c-Myc have given no reproducible results, whereas the profiles of c-Myc-bound proteins and genes identified by NGS and ChIP-on-Chip were significantly different between EMT and non-EMT colon cancer cells, which is under detailed analysis. Our murine orthotopic colon cancer model has to be improved because it presented a technical problem due to necrosis of primary tumor making it difficult to identify cancer cell undergoing EMT.

Free Research Field

腫瘍学