The molecular mechanism of adenomatous polyposis coli-binding protein EB1 in HCC

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430134
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Hokkaido University
• Principal Investigator: NAKANISHI Kazuaki 北海道大学, 医学(系)研究科(研究院), 客員研究員 (80374338)
• Co-Investigator(Kenkyū-buntansha): YOKOO Hideki 北海道大学大学院医学研究科, 消化器外科学分野Ⅰ, 助教 (70399947) ; KAKISAKA Tatsuhiko 北海道大学大学院医学研究科, 消化器外科学分野Ⅰ, 特任研究助教 (40583092) ; KAMIYAMA Toshiya 北海道大学大学院医学研究科, 消化器外科学分野Ⅰ, 准教授 (80322816) ; TAKETOMI Akinobu 北海道大学大学院医学研究科, 消化器外科学分野Ⅰ, 教授 (70363364)
• Co-Investigator(Renkei-kenkyūsha): ORIMO Tatsuya 北海道大学大学院医学研究科, 消化器外科学分野Ⅰ, 助教 (80711861)
• Research Collaborator: FUKUHARA Takasuke ; OHATA Takanori ; TAKAHASHI Hidenori ; KOBAYASHI Nozomi ; MIYOSHI Sayaka ; HORIGOME Masatoshi
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: EB1 / 肝細胞癌 / バイオマーカー / 増殖 / 浸潤 / Dlk-1

Outline of Final Research Achievements

We previously reported that adenomatous polyposis coli-binding protein EB1 (EB1) is overexpressed in HCC tissues by proteomics. In present study, ①EB1 expression significantly correlated with the degree of histological differentiation, AFP, vascular invasion status in HCC patients. Moreover, overall survival and recurrence rate was significantly poor in EB1-high expressed HCC patients. ②EB1 promoted cell proliferation, migration, invasion, tumor growth in HCC cell lines. ③Microarray analysis revealed that EB1 might regulate the expression level of Dlk-1. As a conclusion, EB1 may become a new biomarker of HCC and a potential molecular target of HCC therapy.

Free Research Field

肝癌