Biological implication and significance of microRNA in inflammatory tumorigenesis - histopathological analysis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430143
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor diagnostics
• Research Institution: Fujita Health University
• Principal Investigator: Inada Ken-ichi 藤田保健衛生大学, 医学部, 教授 (70246081)
• Co-Investigator(Kenkyū-buntansha): SHIOGAMA Kazuya 藤田保健衛生大学, 医学部, 助教 (10387699) ; Mizutani Yasuyoshi 藤田保健衛生大学, 医学部, 助教 (10546229)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: microRNA / in situ hybridization / LNA/DNAオリゴプローブ / thyramide / 病理組織切片 / 乳癌 / 扁平上皮癌

Outline of Final Research Achievements

We examined the expressions of miR-199a and miR-200c by in situ hybridization (ISH) in hepatocellular carcinoma, lung cancer and breast cancer. We found that miR-200c expression is increased in EpCAM-positive breast cancer. We plan to reveal the molecular mechanism of the correlation between miR-200c and EpCAM. However, in this study, the expressions were very weak in almost all cases probably due to the deterioration of ISH probes. We need to improve the sensitivity and specificity of our ISH.
Our approach is unique because there are very few researchers who study the inflammation, tumorigenesis and microRNA by pathological methods. We are continuously studying the pathological relationships using a variety of tumors.

Free Research Field

病理学