Development of the novel vector system targeting and identifying cancer stem cells for innovative cancer therapy

2016 Fiscal Year Final Research Report

• Project/Area Number: 25430153
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Tumor therapeutics
• Research Institution: Kagoshima University
• Principal Investigator: Ijichi Nobhiro 鹿児島大学, 医歯学域医学系, 助教 (80380624)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: 腫瘍溶解性ウイルス / がん遺伝子治療 / 癌幹細胞 / アデノウイルス

Outline of Final Research Achievements

In this study, we developed the novel adenoviral vector systems enabling to identify and target the cancer stem cells (CSCs), based on the adenoviral conditional targeting in stem cell (ACT-SC) method that we previouly developed. We constructed the adenoviral vectors carrying two DNA constructs: the regulatory unit expressing the Cre recombinase under the transcriptional control of a various cell-specific promoter and a switching-expression unit that under normal conditions expresses an upstream neo gene but not a reporter gene. Using glioblastoma-derived cancer stem cell population as a model, we then established the screening system of the promoters possibly responsible for cancer stemness by FACS and sphere formation analyses without harmful effects.

Free Research Field

医歯薬学