Physical property of non-coding DNA that is responsible for proper expression of genomic function

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: Hiroshima University
• Principal Investigator: Sakamoto Naoaki 広島大学, 理学(系)研究科(研究院), 准教授 (00332338)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 非コードDNA / インスレーター

Outline of Final Research Achievements

Boundary sequences within non-coding region of genomes are important elements to ensure the proper gene structure and expressions by blocking inappropriate interactions between neighboring genes. In this research, we showed that physical properties of DNA molecule play an important role in the activity of the Ars insulator, which was identified in sea urchin arylsulfatase gene. In analyses of DNA physical properties of artificially synthesized trinucleotide repeats revealed that the property of AAT repeat is similar to that of the Ars insulator. Furthermore, mathematical analysis of ChIP-seq data suggested that surrounding sequence of AAT repeats may have regularly spaced array of nucleosomes. Moreover, during the process of this research, we constructed simple elastic network models for exhaustive analysis of long double-stranded DNA dynamics with sequence geometry dependence.

Free Research Field

分子生物学