cell cycle regulation and genome integrity by novel PCNA unloading mechanism

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430171
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Genome biology
• Research Institution: University of Hyogo
• Principal Investigator: SHIOMI Yasushi 兵庫県立大学, 生命理学研究科, 准教授 (80380567)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: PCNA / RFC複合体 / PCNAローディング / PCNAアンローディング / 細胞周期制御 / ゲノム維持

Outline of Final Research Achievements

PCNA is loaded on chromatin by PCNA loading RFC complex upon initiation of S phase and acts as a platform for a large number of proteins involved not only in DNA replication, but also in replication-linked various processes. To date, three RFC complexes related with PCNA were identified. RFC1-RFC and Ctf18-RFC, function as a PCNA loader, loads PCNA onto DNA. Elg1-RFC also relates with PCNA, but its physiological role in PCNA regulation on chromatin remained unclear.
In this study, PCNA levels on chromatin were increased in Elg1-depleted cells. Various chromatin-associated protein levels on chromatin were affected, and in mitosis, cells with aberrant and lagging chromosomes were frequently detected. These findings suggest that Elg1-RFC has an important role in maintaining chromosome integrity by regulating PCNA levels on chromatin, thereby acting as a PCNA unloader. Our findings demonstrate that Elg1-RFC have unique roles for gemone integrity by controlling PCNA on chromatin.

Free Research Field

分子生物学