2015 Fiscal Year Final Research Report
Study on mammalian spermatozoa freeze-dried to withstand high temperature storage
Project/Area Number |
25430188
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Conservation of biological resources
|
Research Institution | Asahikawa Medical College |
Principal Investigator |
|
Project Period (FY) |
2013-04-01 – 2016-03-31
|
Keywords | フリーズドライ / 精子 / 高温保存 / 室温保存 / 染色体 / DNA / 受精卵 / コメットアッセイ |
Outline of Final Research Achievements |
Chromosomes (DNAs) of freeze-dried mouse spermatozoa could withstand high tempeature storage for short term (3 days to 1 week, 40 or 50°C) or long term (1 to 8 months, 25°C), when the spermatozoa were freeze-dried using EGTA/Tris-HCl-bufferred solution (ETBS) adjusted its pH values to 8.0 to 8.4, or using sodium-free ETBS containing potassium ions.
|
Free Research Field |
細胞遺伝学
|