2014 Fiscal Year Research-status Report
Genome-wide scan for footprints of adaptation/selection and phylogeography structure in the two closely related species of Azuki bean
| Project/Area Number |
25430193
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| Research Institution | Kyushu University |
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Principal Investigator |
FIROUZEH Javadi 九州大学, 持続可能な社会のための決断科学センター, 助教 (70640910)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | Azuki bean / Genetic diversity |
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| Outline of Annual Research Achievements |
Weedy (ノラアズキ), wild (ヤブツルアズキ) and domesticated azuki bean belong to the same biological species (Vigna angularis), but they are different in morphological traits and growing in different habitats. To determine the genetic variation pattern in wild, weedy, and domesticated azuki and their adaptation to different habitats, we performed a field survey, morphological and genetic analyses in this year. In a field survey, we collected 27 populations of weedy and wild azuki populations from Tushima, Japan. The weedy populations has wide distribution pattern in Tsuhima. The habitat and morphological traits were recorded in the field. For genetic diversity, we analyzed the patterns of nucleotide polymorphism of five nuclear genes in the wild and domesticated Azuki beans from different geographical regions. As a result, the four of the five loci surveyed appeared to be evolving primarily under purifying selection, while the remaining locus may have been the subject of positive selection. Outcome of the results will be presented in the conference. In addition, the seeds of wild, weedy, and domesticated azuki bean were obtained from Akita Prefecture University and NIAS, Genebank. Next year, the morphological and genetical analyses (RAD-sequencing) of the selected samples will be reported.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Morphological evaluation and genetic analyses are progressing smoothly. In the field research, surveys and sampling schedule in Korea and Nepal could not be performed because of some official difficulties. However, we requested cooperation with Assoc. Prof. Kentaro Yasuda, Akita Prefecture University for seed sampling. We therefore except the samples will be sufficient for the phylogeographical and genetic analyses using RAD-sequencing in the next fiscal year.
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| Strategy for Future Research Activity |
To identify a large number of SNP markers in the entire genome of wild and domesticated azuki bean using restriction-sit e-associated DNA tags (RAD-tags). The development of these SNP markers as a molecular genetics and genomics resource will be the important step to assess regions of the genome involved in local adaptation and genetic divergence between wild , weedy, and domesticated azuki bean.
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| Causes of Carryover |
In the next fiscal year, morphological evaluation of the collected and germinated wild, weedy, and domesticated azuki beans will be continued. To understand the genetic divergence of the wild, weedy, and domesticated azuki bean, to identify
regions of the genome undergoing selection and local adaptation, RAD (restriction-site-associated DNA) sequencing will be performed. Developing SNP markers generated by RAD-sequencing also will be used to know the phylogeographical relationships of azuki beans populations.
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| Expenditure Plan for Carryover Budget |
The major expenses will be required for the next generation RAD-sequencing using Illumina HighSeq2000. The travel expenses for attending and presenting a lecture in the conference are needed.
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Research Products
(1 results)
