2015 Fiscal Year Annual Research Report

Genome-wide scan for footprints of adaptation/selection and phylogeography structure in the two closely related species of Azuki bean

Research Project

Project/Area Number	25430193
Research Institution	Kyushu University
Principal Investigator	Firouzeh Javadi 九州大学, 学内共同利用施設等, 助教 (70640910)
Project Period (FY)	2013-04-01 – 2016-03-31
Keywords	Genome / Vigna
Outline of Annual Research Achievements	The genome-wide scan of wild and weedy crop relatives is a key to deciphering the genetic basis of their adaptation in the wild and in agronomic contexts following domestication. In this study, Restriction-site-Associated DNA (RAD) tags from 100 accessions (weedy, wild, and cultivated azuki bean), Vigna nepalensis, V. nakashimae, and V. umbellata were sequenced and the sequence variants were analyzed to (i) understand evolutionary relationships between wild relatives and cultivated azuki beans (ii) identify genomic regions contributing to local adaptation and domestication. The neighbor-joining tree indicated that Bhutan and Nepal accessions of wild azuki bean (V. angularis var. nipponensis) and V. nepalensis clustered clearly together in a genetically diverged group. Bhutan and Nepal accessions of cultivated azuki bean clustered together. The weedy Azuki accessions spread among cultivated and wild azuki bean (V. angularis var. nipponensis) which may have played a role in domestication of azuki bean. Further investigation is in progress to test candidate genes that might have contributed to the adaptation in different habitat and genes under selective pressure during domestication.

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