Development of a novel classification method for Aspergillus species based on ribosomal protein biomarkers

2015 Fiscal Year Final Research Report

• Project/Area Number: 25430198
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Conservation of biological resources
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Sato Hiroaki 国立研究開発法人産業技術総合研究所, 環境管理研究部門, 研究グループ長 (70357143)
• Co-Investigator(Kenkyū-buntansha): YAGUCHI TAKASHI 千葉大学, 真菌医学研究センター, 准教授 (60361440) ; TANAKA REIKO 千葉大学, 真菌医学研究センター, 助教 (60143319)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 質量分析 / 微生物同定 / リボソームタンパク質 / アスペルギルス / 真菌

Outline of Final Research Achievements

The purpose of this study is to develop a new method to identify and discriminate Aspergillus fumigatus and relating species (Aspergillus section Fumigati) by mass spectrometry. To make a reliable biomarker list, we have characterized ribosomal subunit proteins of five species of genome sequenced strains of A. fumigatus and relating fungus by mass spectrometry, and verified the translated amino acid sequences registered in public databases. Based on the profiles of the ribosomal protein biomarkers, a phylogenetic tree of clinical and environmental fungal samples could be constructed. These results demonstrated the developed method would be a sufficiently high-throughput technique for rapid discrimination of closely related Aspergillus species.

Free Research Field

機器分析化学、質量分析学