Molecular and functional analysis of translational machenary during oogenesis and development

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440001
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Hokkaido University
• Principal Investigator: Kotani Tomoya 北海道大学, 理学(系)研究科(研究院), 准教授 (70419852)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 翻訳 / mRNA局在 / シス因子 / トランス因子 / 脊椎動物 / 卵母細胞 / RNA顆粒

Outline of Final Research Achievements

In this study, we aimed to elucidate molecular mechanisms of evolutionally conserved control of cyclin B1 mRNA translation to understand the nature of translational machineries by which special and temporal translation is regulated. First, we demonstrated that binding of Pumilio1 to cyclin B1 mRNA is essential for the RNA granule formation, which was found in vertebrate oocytes, and that this Pumilio1-mediated RNA granule formation is important for specially and temporally regulated translational activation of the mRNA. Then, we identified IMP3 protein as a novel factor binding to cycle B1 mRNA, providing novel insight into the translational regulation of the mRNA. Furthermore, we generated transgenic animals visualizing dynamics of actin filaments and obtained results supporting the notion that assembly and depolymerization of actin filaments are involved in the translational regulation.

Free Research Field

分子発生生物学