Identification of the gene responsible for the spermatogenesis defects of a C. elegans mutant

2016 Fiscal Year Final Research Report

• Project/Area Number: 25440002
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Hirosaki University
• Principal Investigator: Ushida Chisato 弘前大学, 農学生命科学部, 准教授 (50250593)
• Project Period (FY): 2013-04-01 – 2017-03-31
• Keywords: リボソーム / U8 snoRNA / 精子形成 / 胚発生 / 受精卵 / 卵殻

Outline of Final Research Achievements

MT16939 is a Caenorhabditis elegans mutant which lacks a region of chromosome IV. There are parts of protein coding genes of unknown function and a small nucleolar RNA (snoRNA). MT16939 shows spermatogenesis defects, embryonic development defects and an egg-shape change. To understand the relationships between the genes and the phenotype alterations, nine novel strains of C. elegans were prepared by microinjection or MosSCI system. The spermatogenesis defects and the embryonic development defects could not be recovered by the introduction of each gene. It is possible that two or three of these genes are required for the recoveries. The change of egg-shape was rescued by the introduction of snoRNA gene.

Free Research Field

分子生物学