2015 Fiscal Year Final Research Report
Reconstitution of mammalian mitochondrial translation system and its application
| Project/Area Number |
25440004
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Research Collaborator |
Akabane Shiori 東京大学, 大学院新領域創成科学研究科, 博士課程大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 翻訳 / ミトコンドリア / 生体外タンパク質合成系 |
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| Outline of Final Research Achievements |
Overall objective of this study is to elucidate the molecular mechanism of translation system in mammalian mitochondria. As a result, we aim to lay the basis for the medical application, such as the design of antibiotics. Utilizing the reconstituted mitochondrial translation system, we have dissected the function of mitochondrial ICT1, a mitoribosomal protein, and yet a member of peptide release factor family. Its substrate specificity as well as the functional domain has been analyzed. We demonstrated the possible engagement of ICT1 in the translation termination at non-standard stop codons AGA and AGG of two mitochondrial ORFs MTCOI and MTND6, respectively. Structures of the mitoribosomal complexes have been determined by cryoEM reconstruction. The structures of PRE- and POST-translocation complex have been determined at high resolution of approximately 4 angstrom.
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| Free Research Field |
生化学、分子生物学
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