2015 Fiscal Year Final Research Report
Genomic instability by Topoisomerase 1 induced by AID-dependent RNA editing
| Project/Area Number |
25440007
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
Kobayashi Maki 京都大学, 医学(系)研究科(研究院), 准教授 (20400690)
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | トポイソメラーゼ１ / AID / ゲノム不安定性 |
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| Outline of Final Research Achievements |
In order to identify the responsive site in Topoisomerase 1 (Top1) mRNA to AID, Top1 3'UTR knockout cells were generated from CH12 B lymphoma cells. Unexpectedly class switch recombination in these knockout cells was not abolished supposing that coding region of Top1 mRNA is the target of AID's regulation.
On the other hand, Top1's involvement in DNA cleavages in immunoglobulin (Ig) gene was further clarified by the proteasome inhibitor and the novel Top1 inhibitors. Additionally by Top1-GFP fusion protein and trapping by GFP, it is elucidated that FACT enrolls as an adopter molecule between Top1 and H3K4me3, modified histone with help of BRG1.
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| Free Research Field |
分子生物学
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