2015 Fiscal Year Final Research Report
Development of novel method of Zero Mode Waveguides for characterization of DNA binding proteins
| Project/Area Number |
25440008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | Kyoto University |
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Principal Investigator |
HAN YONG-WOON 京都大学, 物質－細胞統合システム拠点, 助教 (50566297)
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| Research Collaborator |
IWASA Takuma 京都大学, 大学院生命科学研究科, 大学院生
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| Project Period (FY) |
2013-04-01 – 2016-03-31
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| Keywords | 蛍光１分子イメージング |
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| Outline of Final Research Achievements |
Zero Mode Waveguides (ZMWs) consist of nanosized holes in an aluminum film. Thus, ZMWs enable us the visualization of a single fluorescent molecule under micromolar order of the molecules. We characterized E. coli RuvB motor protein, which promotes Holliday junction DNA branch migration, and SRA, hemi-methyl CpG binding domain. We observed fluorescently labeled RuvBs binding to a RuvA-Holliday junction under various nucleotide conditions and our data suggested that not only ATP binding to RuvB but also ATP hydrolysis by RuvB facilitates a stable RuvA-RuvB-Holliday junction DNA complex formation
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| Free Research Field |
生物学
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