Glycan structural analysis of avian tissues to identify targets of avian influenza viruses

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440014
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Niigata University (2014-2015); The University of Tokyo (2013)
• Principal Investigator: Suzuki Noriko 新潟大学, 自然科学系, 助教 (50401237)
• Co-Investigator(Renkei-kenkyūsha): OKAMATSU Masatoshi 北海道大学, 大学院獣医学研究科, 准教授 (00507163)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: 糖鎖 / 組織 / グライコミクス / 糖タンパク質 / 生物種 / 質量分析 / 高速液体クロマトグラフィー

Outline of Final Research Achievements

Avian influenza viruses (IV) infect host cells by binding to Siaα2-3Gal using virus hemagglutinin (HA). However, it is reported that glycan binding preferences were different between HA from duck IV, which is not pathogenic to the natural host, and HA from chicken IV. These HAs discriminate the glycan structures attaching to Siaα2-3Gal, such as Siaα2-3Galβ1-3GalNAc or Siaα2-3Galβ1-4(Fucα1-3)GlcNAc. These differences of HAs might be generated by adopting specific glycan structures expressed on host cells of different species through the virus infection, growth and accumulated mutations. The differences of glycan structures among these avian species have not been studied well, although glycan structures of them are potentially different from those of mammals. Thus, we tried to establish the method to analyze glycan structures from avian tissues using HPLC and mass spectrometric analysis.

Free Research Field

生化学、糖質科学