The study on inclusive catalytic reaction mechanism of the enzymes used for rare sugar production on the basis of X-ray structures in complexes with rare sugars

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440028
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kagawa University
• Principal Investigator: Yoshida Hiromi 香川大学, 総合生命科学研究センター, 准教授 (10313305)
• Co-Investigator(Kenkyū-buntansha): KAMITORI SHIGEHIRO 香川大学, 総合生命科学研究センター, 教授 (00262246) ; YOSHIHARA AKIHIDE 香川大学, 希少糖研究センター, 助教 (40548765)
• Co-Investigator(Renkei-kenkyūsha): IZUMORI KEN 香川大学, 農学部, 名誉教授 (80035998)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: X線結晶構造解析 / 単糖異性化酵素 / 希少糖 / L-リボースイソメラーゼ / D-タガトース 3-エピメラーゼ / 触媒反応機構 / 酵素・基質複合体 / 微小重力環境タンパク質結晶化

Outline of Final Research Achievements

X-ray structures of L-ribose isomerase from Acinetobacter sp. and Cellulomonas parahominis were determined. On the basis of the wild-type and mutant form structures in complexes with rare sugars, catalytic reaction mechanism of L-RI including ring-opening mechanism of sugar was proposed. Furthermore, mutant structure analysis supported the essentiality of tetramer formation of L-RI for its enzymatic activity.

Free Research Field

構造生物科学、蛋白質工学