Structural study of the peroxisomal membrane protein specific transport sysmem in the peroxisome biogenesis

2015 Fiscal Year Final Research Report

• Project/Area Number: 25440030
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto Sangyo University (2015); Kyushu University (2013-2014)
• Principal Investigator: NIWA Hajime 京都産業大学, 総合生命科学部, 研究員 (30610266)
• Co-Investigator(Kenkyū-buntansha): FUJIKI Yukio 九州大学, 生命防御医学研究所, 特任教授 (70261237)
• Project Period (FY): 2013-04-01 – 2016-03-31
• Keywords: タンパク質複合体 / 結晶構造解析 / 高速AFM / ペルオキシソーム / ミトコンドリア / AAAタンパク質

Outline of Final Research Achievements

To analysis the quality control of mitochondria outer-membrane, we have crystallized Yeast Msp1 and human homologue ATAD1. Yeast Msp1 can be almost solved the structure and revealed the atomic-model which is similar to the p97-D2 domain. Pex7p is the cytosolic receptor protein that is essential for the import of peroxisomal proteins. Newly isolated as a binding protein of Pex7p, termed P7BP2, was found to be a disk-like ring with a central hole under high-speed atomic force microscope (AFM), and implicate that P7BP2 is a novel dynein-type AAA+, whose domains are arranged into a pseudo-hexameric ring structure.

Free Research Field

生物学