2016 Fiscal Year Final Research Report
Molecular mechanism of the sphingolipid-like mycotoxin production in the pathogenic fungi
| Project/Area Number |
25440036
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Osaka Medical College |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
MIYAHARA Ikuko 大阪市立大学, 大学院理学研究科, 准教授 (40271176)
IKUSHIRO Shin-ichi 富山県立大学, 工学部, 教授 (50244679)
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| Project Period (FY) |
2013-04-01 – 2017-03-31
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| Keywords | 可溶性タンパク質 / マイコトキシン産生 / 大腸菌内発現 / 酵母内発現系 / 結晶構造解析 / スフィンゴ脂質 / 難発現性タンパク質 / 活性測定系 |
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| Outline of Final Research Achievements |
We have partially succeeded in constructing the heterologous expression system for both Fum8 and Fum13, which were the key unit of the Fum gene cluster participating to fumonisin B1 biosynthesis. The Fum13p overproduced in Escherichia coli was homogeneously purified, enzymatically characterized and tried to crystallized. New convenient non-RI assay system for the detection of the precursor of FB1 was constructed by combination of the Fum8p-overexpressed yeast microsomes and the purified Fum13p, and substrates synthesized by bacterial SPT. This assay system is useful for the screening of the inhibitor of the fumonisin biosynthesis.
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| Free Research Field |
生化学
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